mouse mast cell protease mmcp  (R&D Systems)

Journal: Marine Drugs

Article Title: Preventive Effect of Depolymerized Sulfated Galactans from Eucheuma serra on Enterotoxigenic Escherichia coli -Caused Diarrhea via Modulating Intestinal Flora in Mice

doi: 10.3390/md19020080

Figure Lengend Snippet: Serological analysis of diarrhea symptoms in mice before and after DESP treatments at doses of 50 mg/kg to 200 mg/kg. ( A ) IgA; ( B ) TNF-α; ( C ) mMCP-1; a–d: bars with different letters represent significant differences ( p < 0.05).

Article Snippet: The ELISA kits of mouse mast cell protease (mMCP)-1 and TNF-αwere obtained from R&D Systems (Minneapolis, MN, USA).

Techniques:

Journal: Mucosal Immunology

Article Title: Orally desensitized mast cells form a regulatory network with Treg cells for the control of food allergy

doi: 10.1038/s41385-020-00358-3

Figure Lengend Snippet: a DNP-IgE bound (None), antigen-stimulated (Activation), or desensitized (Desensitization) bone marrow MCs (BMMCs) were co-cultured with CD4 + T cells and expansion of the Foxp3 + CD4 + T cell population was analyzed by flow cytometry and cell sorting. Numbers indicate percentages of Foxp3 + among all CD4 + T cells. b IgE-bound and desensitized BMMCs and CD4 + T cells were co-cultured under separated conditions in transwells. Percentages of Foxp3 + cells among all CD4 + T cells are shown. c Gene microarray was performed with DNP-IgE bound (None), antigen-stimulated (Activated), and desensitized BMMCs. Shown is a heatmap representative of entities termed cytokines. d Expression of the genes encoding IL-2 and IL-6 was analyzed by quantitative RT-PCR. Data are shown as means ± SEM. Act., activated; Des., desensitized. e IL-2 production was analyzed by ELISA. Data are shown as means ± SEM. *** P < 0.001 (all groups, n = 5). f IL-2 production from hPBMC was analyzed by ELISA. Data are shown as means ± SEM; all groups, n = 3. g Percentages of Foxp3 + cells in coculture of desensitized BMMCs are shown. Isotype or anti-IL-2 antibody was added. h Gene expression of in vivo sorted MCs was analyzed by quantitative RT-PCR. Each result was normalized against the expression of Gapdh . Data are shown as means ± SEM, *** P < 0.01, * P < 0.05.

Article Snippet: Mouse IL-2 production and mouse mast cell protease-1 (mMCP-1) in sera were detected by using the respective ELISA kits (eBioscience, San Diego, CA) in accordance with the manufacturer’s instructions.

Techniques: Activation Assay, Cell Culture, Flow Cytometry, FACS, Microarray, Expressing, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, In Vivo

Journal: Frontiers in Microbiology

Article Title: Recombinant Trichinella pseudospiralis Serine Protease Inhibitors Alter Macrophage Polarization In Vitro

doi: 10.3389/fmicb.2017.01834

Figure Lengend Snippet: Enzyme/substrate used for r Tp -Serpin inhibitory activity assay.

Article Snippet: Mouse mast cell protease-1 (mMCP-1, 3 nM, Sigma Aldrich) , N -succinyl -Ala-Ala-Pro-Phe-pnitroanilide (100 M, Sigma Aldrich).

Techniques: Activity Assay

Journal: Frontiers in Microbiology

Article Title: Recombinant Trichinella pseudospiralis Serine Protease Inhibitors Alter Macrophage Polarization In Vitro

doi: 10.3389/fmicb.2017.01834

Figure Lengend Snippet: Inhibitory activity assay of r Tp -Serpin against different proteases. (A) Inhibitory activity assay of r Tp -Serpin on porcine pancreatic elastase (elastase P), human neutrophil elastase (elastase H), human neutrophil cathepsin G (cathepsin G), and mouse mast cell protease-1 (mMCP-1). (B) Concentration-dependent inhibition profiles of elastase P, elastase H, cathepsin G, and mMCP-1.

Article Snippet: Mouse mast cell protease-1 (mMCP-1, 3 nM, Sigma Aldrich) , N -succinyl -Ala-Ala-Pro-Phe-pnitroanilide (100 M, Sigma Aldrich).

Techniques: Activity Assay, Concentration Assay, Inhibition